Purification and Characterization of Antithrombotics from Syzygium aromaticum (L.) MERR. & PERRY.
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- LEE Jong Im
- Graduate School of Biotechnology, Korea University
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- LEE Hyun Sun
- Institute of Biotechnology, Korea University
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- JUN Woo Jin
- Graduate School of Biotechnology, Korea University
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- YU Kwang Won
- Graduate School of Biotechnology, Korea University
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- SHIN Dong Hoon
- Graduate School of Biotechnology, Korea University
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- HONG Bum Shik
- Graduate School of Biotechnology, Korea University
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- CHO Hong Yon
- Graduate School of Biotechnology, Korea University
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- YANG Han Chul
- Graduate School of Biotechnology, Korea University
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Two antithrombotic polysaccharides with relatively high molecular weight (HMW) and low molecular weight (LMW) were isolated from the flower buds of Syzygium aromaticum (L.) MERR. & PERRY (clove) by anion-exchange chromatography, hydrophobic interaction column chromatography and size exclusion chromatography (LMW: EC-2B-IIIa-2, M.W. ca. 34000; HMW: EC-2C-Ia-2, M.W. ca. 103000). The LMW polysaccharide was mainly composed of Rha, Gal, GalA and Ara (molar %: 24.1, 18.9, 18.0 and 17.9, respectively) with 10.8% of sulfate and 18.2% of protein. The HMW fraction consisted of Ara, Gal, Glc and Rha (molar %: 26.0, 23.7, 17.5 and 12.4, respectively) with 15.4% of sulfate and 8.0% of protein. Both polysaccharides had the backbone of type I rhamnogalacturonan and the side chain of arabinan. Also, most of the sulfates were attached at the position 6 of 3-linked galactosyl residues. Compared to the antithrombotic activity of the HMW fraction (plasma clotting time of 145 s in APTT assay), the LMW fraction displayed a slightly low activity (90 s). However, animal studies indicated that crude LMW polysaccharide did not show acute toxicity, while the acute LD50 of the HMW fraction was approximately 2-fold lower than that of heparin.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 24 (2), 181-187, 2001
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679604613632
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- NII論文ID
- 110003638457
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD3MXhtVSiuro%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 5709981
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- PubMed
- 11217089
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可