Liver Targeting Liposomes Containing β-Sitosterol Glucoside with Regard to Penetration-Enhancing Effect on HepG2 Cells
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- Kawano Kumi
- Institute of Medicinal Chemistry, Hoshi University
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- Nakamura Kouji
- Department of Pharmaceutics, Hoshi University
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- Hayashi Kyoko
- Department of Virology, Toyama Medical and Pharmaceutical University
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- Nagai Tsuneji
- Department of Pharmaceutics, Hoshi University
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- Takayama Kozo
- Department of Pharmaceutics, Hoshi University
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- Maitani Yoshie
- Institute of Medicinal Chemistry, Hoshi University
書誌事項
- タイトル別名
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- Liver Targeting Liposomes Containing .BETA.-Sitosterol Glucoside with Regard to Penetration-Enhancing Effect on HepG2 Cells.
- Liver Targeting Liposomes Containing ベータ Sitosterol Glucoside with Regard to Penetration Enhancing Effect on HepG2 Cells
この論文をさがす
抄録
The aim of this study was to examine the interaction of soybean-derived sterylglucoside (SG) with the human hepatoblastoma cell line HepG2 with regard to the penetration-enhancing effect of β-sitosterol glucoside (Sit-G) to clarify the accumulation of SG-containing liposomes (SG-liposomes) to the liver in vivo. The approach was based on measurement of the association of SG-liposomes labeled with 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiI) in terms of asialoglycoprotein receptor (ASGP-R)-mediated endocytosis, affinity of Sit-G using IAsys and the association of FITC-dextran 4400 (FD-4) increased by Sit-G with the cells. The association of SG-liposomes was decreased by addition of asialofetuin, suggesting that SG-liposomes might be taken up via ASGP-R. Sit-G showed higher affinity with HepG2 cells than HeLa cells, and enhanced the association of FD-4 depending on the incubation time and Sit-G concentrations. Significant positive correlations were found between Sit-G and FD-4 association with the cells, indicating that Sit-G enhanced the drug penetration by distribution in cell membranes. The high degree of liver association of SG-liposomes in vivo might be related to recognition of glucose residues of SG by ASGP-R and to the high affinity and penetration-enhancing effect of Sit-G with hepatocytes.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 25 (6), 766-770, 2002
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390001204628310656
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- NII論文ID
- 110003638754
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD38XkvV2gsrk%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 6175070
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- PubMed
- 12081144
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可