A Novel Oxidized Low-Density Lipoprotein-Binding Protein, Asp-Hemolysin, Recognizes Lysophosphatidylcholine.
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- Kudo Yoichi
- Hygiene Research Laboratory, Sendai Hospital of East Japan Railway Company
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- Ootani Toshihiro
- The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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- Kumagai Takeshi
- The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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- Fukuchi Yuji
- Japanese Red Cross Sendai Hospital
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- Ebina Keiichi
- The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University
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- Yokota Katsushi
- The First Department of Hygienic Chemistry, Tohoku Pharmaceutical University
書誌事項
- タイトル別名
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- Novel Oxidized Low Density Lipoprotein Binding Protein Asp Hemolysin Recognizes Lysophosphatidylcholine
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抄録
Oxidized low-density lipoprotein (Ox-LDL) plays an important role in the initiation and progression of atherosclerosis. Asp-hemolysin, a hemolytic toxin from Aspergillus fumigatus, is a specific, high affinity binding protein for Ox-LDL. We have previously shown that Ox-LDL strongly inhibits the hemolytic activity of Asp-hemolysin, and that the removal of lysophosphatidylcholine (lysoPC) from Ox-LDL abolished the inhibition. In the present study, to clarify the binding mechanism of Asp-hemolysin to Ox-LDL, we investigated the interaction between Asp-hemolysin and lysoPC as a typical lipid moiety of Ox-LDL. Based on western blot analysis, the binding of Asp-hemolysin to LDL, oxidized for different times, depended on the lysoPC content in each Ox-LDL. In addition, the inhibition of lysoPC production in Ox-LDL by phenylmethylsulfonyl fluoride (PMSF) pretreatment of LDL resulted in a marked decrease of Asp-hemolysin binding to PMSF-pretreated Ox-LDL. Furthermore, the binding analysis of Asp-hemolysin to lysoPC using ion-exchange chromatography revealed that Asp-hemolysin directly binds to lysoPC.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 25 (6), 787-790, 2002
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679604904832
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- NII論文ID
- 110003638758
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- NII書誌ID
- AA10885497
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- COI
- 1:CAS:528:DC%2BD38XkvV2gs78%3D
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 6175151
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- PubMed
- 12081148
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可