Involvement of a Novel Mouse Hepatic Microsomal Esterase, ES46.5K, in the Hydrolysis of Phthalate Esters.

• KAYANO Yuichiro

• WATANABE Kazuhito

• MATSUNAGA Tamihide

• YAMAMOTO Ikuo

• YOSHIMURA Hidetoshi

書誌事項

タイトル別名

• Involvement of a Novel Mouse Hepatic Mi

抄録

ES46.5K, a novel esterase from mouse hepatic microsomes (Watanabe K., et al., Biochem. Mol. Biol. Int., 31, 25-30 (1993)), catalyzed hydrolysis of phthalate esters. ES46.5K and mouse hepatic microsomes hydrolyzed diethyl-, dibutyl-, diisobutyl-, dioctyl- and diethylhexyl phthalates, whereas dicyclohexyl- and diphenyl phthalates having ring structure were not hydrolyzed by the enzymes. V_max (μmol/min/mg protein)/K_m (μM) ratios of ES46.5K for diethyl-, dibutyl-, diisobutyl-, dioctyl- and diethylhexyl phthalates were 291, 2786, 565, 51 and 57, respectively, while those of microsomes were 0.58, 0.83, 1.71, 0.05 and 1.10, respectively. The hydrolytic activity of ES46.5K was inhibited by diisopropylfluorophosphate and bis-p-nitrophenylphosphate. These results suggest that ES46.5K has high catalytic activity for phthalate esters and some role in the metabolism of phthalate esters in mice.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 20 (7), 749-751, 1997

  公益社団法人 日本薬学会

キーワード

• hydrolysis
• 46.5-kilodalton esterase (ES46.5K)
• microsome
• esterase
• phthalate ester

詳細情報

• CRID: 1390001204621027968
• NII論文ID: 110003639140
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.20.749
• COI: 1:CAS:528:DyaK2sXkslygtL4%3D
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/09186158
• NDL書誌ID: 4260456
• PubMed: 9255413
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4260456
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/20/7/20_7_749/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1998022221
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可