Expression of Low-Molecular-Weight Kininogen in Mouse Vascular Smooth Muscle Cells.
書誌事項
- タイトル別名
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- Expression of Low-Molecular-Weight Kini
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To determine the existence of the kallikrein-kinin system in vascular wall, the expression of low-molecuar-weith (LMW) kininogen, a precursor protein of kinins, was studied in mouse aortic smooth muscle in vivo or in cultured vascular smooth muscle cells (VSMC) derived from mouse aorta in vitro. Although LMW-kininogen mRNA was undetectable in aortic smooth muscle of untreated mice using either Northern blotting or reverse transcription-polymerase chain reactions (RT-PCR) followed by Southern blotting, administration of lipopolysaccharide (LPS; 1 mg/kg, i.v.) induced the expression of LMW-kininogen mRNA at levels detectable by RT-PCR within 12 h. Cultured VSMC not only expressed LMW-kininogen mRNA at levels easily detectable by RT-PCR, but also secreted LMW-kininogen-like protein that was immunoreactive to anti-mouse LMW-kininogen antibody. These results demonstrate that VSMC are a source of LMW-kininogen in the mouse, and suggest the presence of a local kallikrein-kinin system in vascular tissue. LPS-induced up-regulatin of LMW-kininogen expression suggests a role for vascular LMW-kininogen in tissue trauma or inflammation.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 21 (7), 772-774, 1998
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679600260480
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- NII論文ID
- 110003639457
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- NII書誌ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL書誌ID
- 4538478
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- PubMed
- 9703266
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- PubMed
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- 使用不可