Nucleotide Sequence Specificity in DNA Cleavage Caused by Dihydropyrazines, a New Type of DNA Strand-Cleaving Agent.

• KASHIGE Nobuhiro

• YAMAGUCHI Tadatoshi

• MIAKE Fumio

• WATANABE Kenji

抄録

The specificity of the nucleotide sequence of DNA strand cleavage sites produced by dihydropyrazines, a new type of DNA strand-cleaving agent, was studied. Biotin-5'-end-labeled PCR-amplified DNA restriction fragments of different defined nucleotide sequences were prepared from plasmid pBR322, and reacted with dihydropyrazines in the presence of Cu²⁺. After being heated with aqueous piperidine, the DNA products were analyzed on polyacrylamide gels. The most preferentially cleaved sites induced by dihydropyrazines were at purine/pyrimidine-guanine (5'→3') sequences. The purine/pyrimidine-adenine, pyrimidine-pyrimidine and purine-pyrimidine (5'→3') sequences were more resistant to attack by these dihydropyrazines. The side chains of the dihydropyrazine skeleton greatly affected the DNA strand-cleaving activity, and also, to some extent, the nucleotide sequence-specificity in the DNA strand-cleavage.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 23 (11), 1281-1286, 2000

  公益社団法人 日本薬学会

キーワード

• dihydropyrazine
• nucleotide sequence-specificiy
• DNA cleavage
• plasmid pBR322

詳細情報

• CRID: 1390001204628171520
• NII論文ID: 110003639872
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.23.1281
• COI: 1:CAS:528:DC%2BD3cXnvVyhsb8%3D
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/09186158
• NDL書誌ID: 5539356
• PubMed: 11085352
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5539356
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/23/11/23_11_1281/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可