Radioimmunoassay of Conjugated 1.BETA.-Hydroxycholic Acid in Dried Blood Spots for Diagnosis of Congenital Biliary Atresia.

• IKEGAWA Shigeo

• MURAI Tsuyoshi

• MATSUI Akira

• TOHMA Masahiko

Abstract

Radioimmunological measurement of conjugated 1β-hydroxycholic acid in dried blood spots obtained from newborns at 4-5 d after birth has been carried out to investigate a diagnostic utility for congenital biliary atresia. The method allowed the determination of this bile acid, with a detection limit of 50 pg in one disc (3 mm i.d.), and showed good reproducibility with inter- and intra-assay coefficients with variations of 3.5-7.7% and 6.3-8.8%. With the analysis, a relatively high concentration of this substance was observed in the patients, as compared to normal subjects, averaging 1.63 ng/disc in the former and 0.97 ng/disc in the latter.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 17 (1), 5-8, 1994

  The Pharmaceutical Society of Japan

Keywords

• radioimmunoassay
• conjugated 1β-hydroxycholic acid
• bile acid
• dried blood disc
• gas chromatography-mass spectrometry
• congenital biliary atresia

Details

• CRID: 1390001204622913408
• NII Article ID: 110003640386
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.17.5
• ISSN: 13475215
• ISSN: 09186158
• PubMed: 8148816
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/17/1/17_1_5/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1995082284
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed