A Non-.ALPHA.-like DNA Polymerase from the Hyperthermophilic Archaeon Pyrococcus furiosus.

• 今村 光雄

• 上森 隆司

• 加藤 郁之進

• 石野 良純

抄録

We identified a DNA polymerase with properties that differed from those of α-like DNA polymerase (Pol I), in Pyrococcus furiosus, a hyperthermophilic archaeon. The novel DNA polymerase (Pol II) was partially purified and characterized. The DNA polymerizing activity of Pol II was relatively sensitive to dideoxythymidine-triphosphate (ddTTP) and it was inhibited by N-ethylmaleimide, but not by aphidicolin. Activity staining gel electrophoresis showed that the DNA polymerizing activity was derived from a polypeptide with a molecular weight of 130000 on sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The DNA elongation ability of Pol II using a natural DNA template was much lower than that of Pol I from this organism and DNA synthesis of Pol II seems to be non-processive.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 18 (12), 1647-1652, 1995

  公益社団法人 日本薬学会

キーワード

• DNA polymerase
• thermophilic archaeon
• Pyrococcus furiosus

詳細情報

• CRID: 1390282679599449984
• NII論文ID: 110003640774
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.18.1647
• COI: 1:CAS:528:DyaK28XisFSisg%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• ISSN: http://id.crossref.org/issn/09186158
• PubMed: 8787781
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/18/12/18_12_1647/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可