Isolation and Characterization of a cDNA Encoding a Human TFIID Subunit Containing a Variety of Putative Structural Motifs Including Direct Repeats.

• KUZUHARA Takashi

• HORIKOSHI Masami

抄録

A cDNA encoding human TFIID subunit p30β (hp30β) was isolated and sequenced. Comparison of the deduced 211 amino acid (aa) sequence with that of Drosophila melanogaster p30β (dp30β) showed 45% identity in overall length, and subunits with highly conserved C-terminal (81% identity) and less conserved N-terminal (21% identity) regions. Three acidic and basic regions were alternately placed. Potent target sites for protein kinases and a putative nuclear localization signal (NLS) were located in the N-terminal region. Interestingly, analysis of the aa sequence of p30β led us to find several interesting structural motifs/regions including 1) direct repeats, 2) a region similar to histone H4 between the two direct repeats and 3) acidic and hydrophobic aa surfaces in the potent helical region. Northern blot analysis showed ubiquitous expression of mRNA corresponding to hp30β.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 19 (1), 122-126, 1996

  公益社団法人 日本薬学会

キーワード

• transcription initiation
• transcriptional regulation
• acidic, hydrophobic surface
• direct repeat
• histone H4 similarity

詳細情報

• CRID: 1390282679598256128
• NII論文ID: 110003641071
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.19.122
• ISSN: 13475215
• ISSN: 09186158
• PubMed: 8820923
• Web Site: http://www.jstage.jst.go.jp/article/bpb1993/19/1/19_1_122/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可