高速液体クロマトグラフィーによる養殖魚中の水産用医薬品の一斉分析

• 堀江 正一

• 星野 庸二

• 能勢 憲英

• 岩崎 久夫

• 中澤 裕之

書誌事項

タイトル別名

• Simultaneous determination of antibiotics and synthetic antibacterials in fish by high performance liquid chromatography.

抄録

A high performance liquid chromatographic method (HPLC) was developed for the simultaneous determination of oxytetracycline (OTC), chlortetracycline (CTC), chloramphenicol (CP), sulfamonomethoxine (SMMX), sulfadimethoxine (SDMX), furazolidone (FZ) and difurazone (DFZ) in cultured fish. The drugs were extracted from fish with 0.5% metaphosphoric acid-methanol (8 : 2), and the extract was filtered and concentrated. The concentrated solution was passed through a Sep-pak C₁₈ cartridge. The cartridge was washed with water and eluted with methanol. The eluate was evaporated to dryness, and the residue was dissolved in acetonitrile-water (3 : 7). The sample solution was analysed by HPLC using a Nucleosil 5 C₁₈ column as a column and acetonitle 0.05 M monobasic sodium phosphate (35 : 65), pH 2.5 as a mobile phase. The average recoveries of OTC, CTC, CP, SMMX, SDMX and FZ from rainbow trouts, yellowtails and red sea breams fortified at 1.0 μg/g were 68.9-91.7%. The limits of detection were 0.05 μg/g for OTC, CP, SMMX, SDMX and FZ, 0.1 μg/g for CTC and 1.0 μg/g for DFZ.

収録刊行物

• 衛生化学

  衛生化学 31 (6), 371-376, 1985

  公益社団法人 日本薬学会

キーワード

• oxytetracycline
• chlortetracycline
• chloramphenicol
• sulfamonomethoxine
• sulfadimethoxine
• furazolidone
• difurazone
• fish
• HPLC

詳細情報

• CRID: 1390001204496840704
• NII論文ID: 110003643262
• NII書誌ID: AN00021540
• DOI: 10.1248/jhs1956.31.371
• COI: 1:CAS:528:DyaL28XktFOktr0%3D
• ISSN: 0013273X
• Web Site: http://www.jstage.jst.go.jp/article/jhs1956/31/6/31_6_371/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1986162489
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可