キャピラリー電気泳動法による胃腸薬中のロートエキスの迅速定量

• 小里 一友

• 三方 比奈子

• 鶴森 敏樹

書誌事項

タイトル別名

• A Rapid Determination Method for Scopolia Extract in Gastrointestinal Drugs by Copillary Electrophoresis
• キャピラリー デンキ エイドウホウ ニ ヨル イチョウヤク チュウ ノ ロートエキス ノ ジンソク テイリョウ

抄録

A rapid and simple determination of tropane-alkaloids (hyoscyamine, scopolamine) in gastrointestinal drugs was investigated by capillary electrophoresis. Micellar electrokinetic capillary chromatography (MEKC) using a fused silica capillary (560 mm×0.075 mm i.d.) in 0.1 M SDS 20 mM borate buffer (pH 10)/acetonitrile (97 : 3) gave complete separation of the two alkaloids within 40 min. A sample solution was introduced by pressure method (50 mbar, 3.7 s), separation conditions applied voltage 15 kV and on-column detection was performed at 210 nm. Calibration curves for hyocyamine and scopolamine showed a good linearity in the range of 4-12μg/ml(r=0.9970) and 390-1150 ng/ml(r=0.9976). The present method is applicable to the simple and rapid determination of hyoscyamine and scopolamine in commercial gastrointestinal drugs.

収録刊行物

• 薬学雑誌

  薬学雑誌 119 (11), 868-879, 1999

  公益社団法人 日本薬学会

キーワード

• capillary electrophoresis (CE)
• hyoscyamine
• scopolamine
• Scopolia extract
• Scopolia japonica MAXIM
• gastrointestinal drug

詳細情報

• CRID: 1390282681130755840
• NII論文ID: 110003648730
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi1947.119.11_868
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 4890929
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4890929
• Web Site: https://www.jstage.jst.go.jp/article/yakushi1947/119/11/119_11_868/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2000088023
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