書誌事項
- タイトル別名
-
- A Sensitive Fluorometric Assay for Dihydrodiol Dehydrogenase
- ジヒドロジオール ダツ スイソ コウソ カッセイ ノ コウカンド ケイコウ ソ
この論文をさがす
抄録
Dihydrodiol dehydrogenase (DD) oxidizes naphthalene dihydrodiol to 1, 2-dihydroxynaphthalene, which is immediately autoxidized to 1, 2-naphthoquinone. Here we established a fluorometric assay for the enzyme, which is based on the conversion of 1, 2-naphthoquinone to fluorescent compounds by reacting with ethylenediamine. The formed fluorescent compounds were synthetically identified as 6-(2-aminoethylamino)benzo[ƒ]quinoxaline and 2, 6- or 3, 6-bis(2-aminoethylamino)benzo[ƒ]quinoxaline, which showed the same fluorescence at 550 nm at an excitation wavelength of 420 nm. This method provides a 9000-fold increase in sensitivity over a currently available assay which measures the change in the absorbance of a cofactor, NADPH. Since this simple and sensitive method allowed many samples to be assayed simultaneously, we applied it to the analysis of multiple forms of DD, separated by an anion-exchange chromatography, from six human liver specimens.
収録刊行物
-
- 薬学雑誌
-
薬学雑誌 117 (3), 167-177, 1997
公益社団法人 日本薬学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001206154350208
-
- NII論文ID
- 110003649078
-
- NII書誌ID
- AN00284903
-
- ISSN
- 13475231
- 00316903
-
- NDL書誌ID
- 4174533
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可