Counter Alternative Current Chromatographyの高純度レシチン(卵製)調製への応用

DOI Web Site Web Site 被引用文献5件

書誌事項

タイトル別名
  • Application of Counter Alternative Current Chromatography to Preparation of High Grade Lecithin (Egg)
  • Counter Alternative Current Chromatogra

この論文をさがす

抄録

High grade lecithin (egg) was prepared by counter alternative current chromatography (CACC) with different partition between two immiscible liquids for chromatographic purification of various substances. The most distinctive feature of the CACC is a system sending alternately upper and lower phase as mobile phase to the column. Consequently, without a long size column, separation is proceeded by moving the solutes back and forthe within the column assembly. The commercial reagent grade lecithin developed more than nine spots on a silica gel thin layer chromatography (TLC) plate and was identified to be a mixture containing ca. 21% of lecithin. The effect of the numbers for switchover of mobile phase and the amount of sample on the purification of lecithin were investigated. The phase system used for the purification is composed of n-heptan-n-buthanol-chloroform-methanol-60% acetic acid (3 : 2 : 2 : 3 : 5, v/v). The preparation of high grade lecithin forming stable liposome was obtained by two or four times switchovers of the mobile phase. The present method is applicable to the preparative purification of natural products, particularly polar compounds, by using the system with liquid two phases highly efficiently.

収録刊行物

  • 薬学雑誌

    薬学雑誌 109 (3), 168-171, 1989

    公益社団法人 日本薬学会

被引用文献 (5)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ