Spectrophotometric and Fluorimetric Determination of Hexamine in Pure Form and Its Pharmaceutical Formulation

• Balasubramanian Maruthamuthu

  Organic Chemistry Division, Central Leather Research Institute

• Thennarasu Sathiah

  Organic Chemistry Division, Central Leather Research Institute

• Sudhakaran Thankiah

  Jonaki, BRIT, CCMB-Campus

• Perumal Paramasivam Thirumalai

  Organic Chemistry Division, Central Leather Research Institute

抄録

Ethyl acetoacetate and methyl acetoacetate have been tested for the determination of hiprex (methenamine hippurate). Both the esters react with hexamine and hiprex forming fluorescent yellow Hantzsch esters. The products show absorption maximum at 370 nm and a fluorescence emission maximum at 465 nm in aqueous n-propanol. Important parameters such as pH, temperature, mole ratio and time of reaction have been optimized. The co-efficient of variation for inter and intra-day precisions were less than 1%. The chromogens show linearity in the concentration ranges of 2.0—35.0 μg ml⁻¹ and 0.6—40.0 μg ml⁻¹ for the spectrometric and fluorimetric determinations of hiprex, respectively.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ

  Ｂｉｏｌｏｇｉｃａｌ　＆　Ｐｈａｒｍａｃｅｕｔｉｃａｌ　Ｂｕｌｌｅｔｉｎ 26 (8), 1211-1214, 2003

  公益社団法人 日本薬学会

キーワード

• hiprex
• hexamine
• fluorescence
• spectrophotometry
• methyl acetoacetate
• ethyl acetoacetate

詳細情報

• CRID: 1390001204624446976
• NII論文ID: 110003655081
• NII書誌ID: AA10885497
• DOI: 10.1248/bpb.26.1211
• COI: 1:STN:280:DC%2BD3szovFWisA%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL書誌ID: 6620575
• PubMed: 12913281
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6620575
• Web Site: http://www.jstage.jst.go.jp/article/bpb/26/8/26_8_1211/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003291226
• 本文言語コード: en
• データソース種別:
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  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可