A sensitive method for the fluorometric analysis of phenylthiohydantoin amino acids using hypochlorite-thiamine reagent.

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A sensitive method for the detection and determination of phenylthiohydantoin amino acids using hypochlorite-thiamine reagent is described. Phenylthiohydantoin amino acids were developed on a Kieselgel plate and N-chlorinated on the plate by spraying sodium hypochlorite. Excess hypochlorite was removed by aeration and the plate was sprayed with the alkaline thiamine reagent. The chlorinated thiohydantoin gave and intensely fluorescent spot. An application of this method to Edman degradation is also described.

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