cDNA Isolation and Functional Expression of Myrcene Synthase from Perilla frutescens
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- Hosoi Madoka
- Graduate School of Pharmaceutical Sciences, Kyoto University
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- Ito Michiho
- Graduate School of Pharmaceutical Sciences, Kyoto University
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- Yagura Toru
- Graduate School of Pharmaceutical Sciences, Kyoto University
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- Adams Robert Phillip
- Biology Department, Baylor University
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- Honda Gisho
- Graduate School of Pharmaceutical Sciences, Kyoto University
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Abstract
cDNA cloning of a monoterpene synthase from Perilla frutescens whose steam-distilled oil contains 92.9% perillaketone, was performed by the PCR method using primers designed based on limonene synthase. The full-length nucleotide sequence of this cDNA consisted of 1978 bp including a 1827-bp translational region encoding a deduced protein of 608 amino acids, which was similar to that of limonene synthase from P. frutescens (85% identity). Functional expression of this clone in Escherichia coli yielded an active monoterpene synthase enzyme, which converted geranyl diphosphate into 53.8% myrcene, 20.9% sabinene, 19.8% linalool and 5.5% limonene. As for the extraction of reaction products, we performed SPME (solid phase micro extraction) as well as conventional solvent extraction, and compared these two extraction methods.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 27 (12), 1979-1985, 2004
The Pharmaceutical Society of Japan
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Keywords
Details 詳細情報について
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- CRID
- 1390001204627436416
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- NII Article ID
- 110003665744
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 7172790
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- PubMed
- 15577217
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed