形態的形質およびＲＡＰＤ法によるヤムイモ（Ｄｉｏｓｃｏｒｅａ　ｓｐｐ．）の種の分類と系統の区分

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  • Classification of Yams (Dioscorea spp.) Based on Morphological Characters and RAPD Method.
  • ケイタイテキ ケイシツ オヨビ RAPDホウ ニ ヨル ヤムイモ Dioscorea spp ノ タネ ノ ブンルイ ト ケイトウ ノ クブン

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Water yam (D. alata), Chinese yam (D. opposita) and jinen-jo (D. japonica) were discriminated by morphological characters and random amplified polymorphic DNA (RAPD) method.<BR>Water yams could be classified on the characteristics of leaves, stems and aerial tubers. It was difficult to distinguish some strains of Chinese yam from jinen-jo because of the similarity of the morphological characteristics. However, jinen-jo was distinguished from Chinese yams by using RAPD method. This suggests that the RAPD method is useful for the identification of species of yams.<BR>The dendrogram based on the RAPD method corresponded with cluster analysis based on the morphological characteristics among the three species of yams. On the other hand, water yams were classified into three groups by cluster analysis based on the morphological characteristics, but were classified into five groups by the RAPD method. In RAPD analysis, morphological characteristics of strains may differ from each other. Therefore to classify yam strains using the RAPD method, it is necessary to use RAPD marker related to the specific characteristics.<BR>The appearance of anthocyanin on the leaf axil or petiole could be used as an indicator for classification in the strains of water yam and jinen-jo roughly. However, circumstantial classification of the strains should be combined with use of other indicators.<BR>Based on both analysis of morphological characteristics and the RAPD method in water yam, the strains from Indonesia were found to be similar to Japanese, Micronesian and Oceanian strains. This result suggests that there is a wide variation in water yam in Indonesia. The strains of water yam in Japan displayed similar characteristics to those of the strains of Indonesia unlike those of Micronesia and Oceania. These findings suggest that Japanese strains were originated from South-East Asia.

収録刊行物

  • 熱帯農業

    熱帯農業 44 (4), 229-237, 2000

    日本熱帯農業学会

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