Purification and properties of δ-aminolevulinic acid dehydratase from radish cotyledons :
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- Shibata,Hitoshi
- Laboratory of Biochemistry, college of Agriculture, Shimane University
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- Ochiai,Hideo
- Laboratory of Biochemistry, college of Agriculture, Shimane University
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抄録
δ-Aminolevulinic acid dehydratase (5-aminolevulinate hydro-lyase, EC; 4.2.1.24) was purified from greening radish cotyledons. The final product was homogeneous on polyacrylamide disc gel electrophoresis and had a molecular weight, estimated by gel filtration, of 282,000 daltons. The enzyme seems to require magnesium ion as well as sulfhydryl compounds for maximum activity. EDTA and a low concentration of zinc ion markedly inhibited the activity. The optimum pH was 8.0; the Km value for δ-aminolevulinic acid was 3.85×10^<-4> M. Levulinic acid was a competitive inhibitor of the enzyme, with a Ki of 2.14×10^<-4> M. These properties were compared with those of microorganism and animal δ-aminolevulinic acid dehydratases.
収録刊行物
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- Plant and cell physiology
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Plant and cell physiology 18 (2), 421-429,
Japanese Society of Plant Physiologists
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詳細情報 詳細情報について
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- CRID
- 1543950420080666624
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- NII論文ID
- 110003717260
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- NII書誌ID
- AA0077511X
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- 本文言語コード
- en
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- データソース種別
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- NDL-Digital
- CiNii Articles