Pumpkin (Cucurbita sp.) seed globulin III. Comparison of subunit structures among seed globulins of various Cucurbita species and characterization of peptide components :

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Various Cucurbita seed globulins showed patterns similar to one another on SDS-gel electrophoresis, α and β bands for unreduced globulins and γ,γ',δ and δ' bands for reduced ones. On gel electrophoresis in 6 M urea, reduced globulin gave two acidic and two basic bands. These corresponded to y and γ' chains and δ_1 and δ_2 chains, respectively, identified by two-dimensional urea-SDS gel electrophoresis. The compositions of the α and β subunits were proposed.

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