Direct demonstration of the involvement of chloroplasts in the rapid light-induced potential change in tonoplast-free cells of Chara australis. Replacement of Chara chloroplasts with spinach chloroplasts :
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- Tazawa,Masashi
- Department of Botany, Faculty of Science, University of Tokyo
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- Shimmmen,Teruo
- Department of Botany, Faculty of Science, University of Tokyo
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To directly demonstrate the involvement of chloroplasts in the rapid light-induced potential change we removed chloroplasts by centrifuging tonoplast-free cells of Chara australis. Chloroplast-free cells showed no signs of a potential change on illumination. When a chloroplast-free Chara cell was perfused with a suspension of chloroplasts isolated from other cells of Chara australis, it regained its ability to generate the rapid potential change induced by light. Chloroplasts were isolated from spinach leaves by a method which preserves the chloroplast envelope; thus ensuring the ability to assimilate CO_2. When these chloroplasts were introduced into a transparent Chara cell containing no chloroplasts, normal rapid hyperpolarization was induced on illumination. When spinach chloroplasts were treated with a medium of very low osmotic potential, no O_2 evolution was detected, but even with these chloroplasts rapid light-induced hyperpolarization was observed. We concluded that chloroplasts are essential for the rapid light-induced potential change and that chloroplasts can be replaced with those of another species, so far as the light-induced potential change is concerned.
収録刊行物
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- Plant and cell physiology
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Plant and cell physiology 21 (8), 1527-1534,
Japanese Society of Plant Physiologists
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詳細情報 詳細情報について
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- CRID
- 1544231895057471616
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- NII論文ID
- 110003717944
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- NII書誌ID
- AA0077511X
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- 本文言語コード
- en
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- データソース種別
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- NDL-Digital
- CiNii Articles