Demonstration of the K^+ channel in the plasmalemma of tonoplast-free cells of Chara australis :

• Tazawa,Masashi

  Department of Botany, Faculty of Science, University of Tokyo

• Shimmmen,Teruo

  Department of Botany, Faculty of Science, University of Tokyo

抄録

Tonoplast-free and ATP-less internodal cells of Chara australis were made by perfusing cells internally with a medium containing ethyleneglycol-bis-(β-aminoethylether)N, N'-tetraacetic acid, hexokinase and glucose. Such a cell showed rapid light-induced potential change when it was stained with neutral red (5). The rapid light-induced potential change was inhibited by tetraethylammonium, a known inhibitor of the K^+ channel in nerve cells. Tetraethylammonium acted both from the inside of the membrane and from the outside with similar effectiveness. Nonyl-triethylammonium inhibited the light response far more effectively than did tetraethylammonium. The results suggest the existence of a K^+ channel which is activated by light in the Chara membrane.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 21 (8), 1535-1540,

  Japanese Society of Plant Physiologists

キーワード

• Chara
• K^+_channel
• Membrane potential
• Tetraethylammonium
• Tonoplast-free cell

詳細情報

• CRID: 1543105995150628992
• NII論文ID: 110003717945
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10768095
• 本文言語コード: en
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  • NDL-Digital
  • CiNii Articles