Intracellular Ca^<2+> Concentration and Cytoplasmic Streaming in Vallisneria Mesophyll Cells :

  • Takagi,Shingo
    Department of Biology, College of General Education, Osaka University
  • Nagai,Reiko
    Department of Biology, College of General Education, Osaka University

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Intracellular Ca^<2+> concentration regulating the cytoplasmic streaming in Vallisneria mesophyll cells was estimated. The leaf segment was cut open at the middle of the mesophyll cell layers and the exposed mesophyll cells were treated with test solutions of various Ca^<2+> concentrations in the dark. This allowed A23187, a calcium ionophore, to exert its full effect on the cell membrane. The streaming was induced or maintained in solutions which contained Ca^<2+> at lower than 10^<-6>M. However, Ca^<2+> at concentrations higher than 10^<-5>M had a definite, inhibitory effect. The induction and cessation of streaming could be repeated by alternately changing the solutions.

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