Isolation of a Gene for a Metallothionein-Like Protein from Soybean :

• Kawashima,Ichiro

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• Inokuchi,Yoshio

  Department of Molecular Biology, Keio University School of Medicine:(Present)Department of Biosciences, Teikyo University

• Chino,Mitsuo

  Department of Agricultural Chemistry, Faculty of Agriculture, The University of Tokyo

• Kimura,Masami

  Department of Experimental Toxicology, National Institute of Industrial Health

• Shimizu,Nobuyoshi

  Department of Molecular Biology, Keio University School of Medicine

抄録

Using a synthetic oligonucleotide that corresponded to the consensus nucleotide sequence of the N-terminal region of mammalian metallothionein as probe, we isolated a cDNA clone from a soybean library. The clone had an ORF that encode a protein of 79 amino acids which showed significant homology to both N- and C-terminal regions of mammalian and Neurospora crassa metallothioneins.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 32 (6), 913-916,

  Japanese Society of Plant Physiologists

キーワード

• CDNA
• Cysteine cluster
• Metallothionein
• Oligonucleotide probe
• Soybean (Glycine max. L. Merr.)

詳細情報

• CRID: 1543950420081071744
• NII論文ID: 110003720004
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10770758
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles