Structure and Expression of Chalcone Synthase Gene in Carrot Suspension Cultured Cells Regulated by 2,4-D :
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- OZEKI,Yoshihiro
- Department of Biology, College of Arts and Sciences, The University of Tokyo
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- DAVIES,Eric
- School of Biological Sciences, University of Nebraska-Lincoin
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- TAKEDA,Junko
- Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University
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抄録
Two nearly full-length chalcone synthase (EC 2.3.1.74) cDNAs, designated CHS2 and CHS9, were isolated from a cDNA library of mRNA prepared from anthocyanin-synthesizing cells of carrot suspension cultures and showed 98% nucleotide sequence identity to each other. The genomic sequence (gCHS2), which corresponds to CHS2, was isolated from a carrot genomic library. Primer extension identified the transcription start site of gCHS2 at 72 bp upstream from the initiation codon ATG, and showed that gCHS2 was activated during anthocyanin synthesis. A chimeric gene carrying the gCHS2 putative promoter sequence from - 2,357 bp to +92 bp connected to the firefly luciferase gene and Nos terminator was introduced by electroporation into protoplasts prepared from anthocyanin-synthesizing carrot cells and the effects of light and 2,4-D on the promoter activity were examined by a transient assay for luciferase activity. Luciferase activity was induced by light exposure in the medium lacking 2,4-D but suppressed by the addition of 2,4-D and/or dark treatment.
収録刊行物
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- Plant and cell physiology
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Plant and cell physiology 34 (7), 1029-1037,
Japanese Society of Plant Physiologists
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詳細情報
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- CRID
- 1541417145290737536
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- NII論文ID
- 110003720363
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- NII書誌ID
- AA0077511X
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- 本文言語コード
- en
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- データソース種別
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- NDL-Digital
- CiNii Articles