Microgravity and Clinorotation Cause Redistribution of Free Calcium in Sweet Clover Columella Cells : ENVIRONMENTAL AND STRESS RESPONSES :

• Hilaire,Emmanuel

  Kansas state University, NASA Secialized Center of Research and Training in Gravitational Biology, Division of Biology

• Paulsen,Avelina Q.

  Kansas state University, NASA Secialized Center of Research and Training in Gravitational Biology, Division of Biology

• Brown,Chiristopher S.

  Dynamac Corporation, Plant Space Biology Laboratory

• Guikema,James A.

  Kansas state University, NASA Secialized Center of Research and Training in Gravitational Biology, Division of Biology

抄録

In higher plants, calcium redistribution is believed to be crucial for the root to respond to a change in the direction of the gravity vector. To test the effects of clinorotation and microgravity on calcium localization in higher plant roots, sweet clover (Melilotus alba L.) seedlings were germinated and grown for two days on a slow rotating clinostat or in microgravity on the US Space Shuttle flight STS-60. Subsequently, the tissue was treated with a fixative containing antimonate (a calcium precipitating agent) during clinorotation or in microgravity and processed for electron microscopy. In root columella cells of clinorotated plants, antimonate precipitates were localized adjacent to the cell wall in a unilateral manner. Columella cells exposed to microgravity were characterized by precipitates mostly located adjacent to the proximal and lateral cell wall. In all treatments some punctate precipitates were associated with vacuoles, amyloplasts, mitochondria, and euchromatin of the nucleus. A quantitative study revealed a decreased number of precipitates associated with the nucleus and the amyloplasts in columella cells exposed to microgravity as compared to ground controls. These data suggest that roots perceive a change in the gravitational field, as produced by clinorotation or space flights, and respond respectively differently by a redistribution of free calcium.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 36 (5), 831-837,

  Japanese Society of Plant Physiologists

キーワード

• Calcium
• Melilotus alba L.
• Microgravity
• Root
• Spaceflight

詳細情報

• CRID: 1540291245383947136
• NII論文ID: 110003720670
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771649
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles