Trans-Kingdom Conjugation between Agrobacterium tumefaciens and Saccharomyces cerevisiae, a Bacterium and a Yeast :

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For conjugation between prokaryotic Agrobacterium tumefaciens and eukaryotic Saccharomyces cerevisiae, we constructed two novel conjugative plasmids. A. tumefa-ciens transmitted the plasmids to S. cerevisiae with the aid of tra genes on a helper plasmid. The transmitted plasmids retained their original structure and function in transcon-jugant yeasts. The presence of Ti plasmid barely affected the trans-kingdom conjugation.

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