Emergence of Proteases in Germinating Cucumber Cotyledons and Their Roles in the Two-Step Degradation of Storage Protein :

• Yamauchi,Yasuo

  Department of science of Bioiogical Resources, Graduate school of science and Technoiogy, Kobe University

• Sugimoto,Toshio

  Faculty of Agriculture, Kobe University

• Sueyoshi,Kuni

  Faculty of Agriculture, Kobe University

• Oji,Yoshikiyo

  Faculty of Agriculture, Kobe University

抄録

The degradation of storage protein in germinating cu-cumber seeds was shown to proceed via two distinct steps. First, several proteases with acidic isoelectric points (pls) were involved in solubilization and partial degradation of 11S globulin. Treatment of seedlings with cycloheximide inhibited this step and the expression of these proteases. Thus, the first step appeared to be governed by these pro-teases, which were synthesized de novo after imbibition. The first step was observed in dark-grown cotyledons, but the complete degradation of 11S globulin did not occur in the absence of illumination. An additional protease, with a pI of 4.5, was induced by illumination, and it was involved in the further cleavage of the partially degraded products of 11S globulin. Thus, the complete degradation of the storage protein proceeded via a two-step process in illuminated germinating seedlings. Light is needed to induce the second step in the degradation of 11S globulin that supplies the nitrogen required for development of the photosynthetic ap-paratus in the greening cotyledon.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 37 (3), 279-284,

  Japanese Society of Plant Physiologists

キーワード

• Cucumber-1IS Globulin-Light dependentproteolysis-Protease.

詳細情報

• CRID: 1542824520174369536
• NII論文ID: 110003720818
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771846
• 本文言語コード: en
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  • CiNii Articles