cDNA Cloning of a Wounding-Inducible Gene Encoding a Plastid ω-3 Fatty Acid Desaturase from Tobacco :

• Hamada,Tatsurou

  Department of Biology, Faculty of Science, Kyushu University

• Nishiuchi,Takumi

  Department of Biology, Faculty of Science, Kyushu University

• Kodama,Hiroaki

  Department of Biology, Faculty of Science, Kyushu University

• Nishimura,Mitsuo

  Department of Biology, Faculty of Science, Kyushu University

• Iba,Koh

  Department of Biology, Faculty of Science, Kyushu University

抄録

A cDNA encoding the plastid ω-3 fatty acid desatura-se was isolated from a tobacco (Nicotiana tabacum cv. SR1) leaf cDNA library. The amino terminal extension of the deduced amino acid sequence of this clone had a characteristic feature of the transit peptides of plastid-destined proteins. Northern analysis indicated that the mRNA corresponding to this cDNA was present in leaves, but was not detected in roots. Responses to wounding of the plastid and microsome ω-3 desaturase genes were investigated in tobacco leaves. The mRNA level of the plastid ω-3 desatu-rase gene increased to about 2-fold that of unwounded controls at 12 h after a wounding treatment. On the other hand, the mRNA level of the microsome ω-3 desaturase gene remained constant in the wounded leaves. Linolenic acid contents of major leaf polar lipids increased by wounding. These results indicate that wounding enhances the accumulation of the plastid ω-3 desaturase mRNA, and incteases the conversion of linoleic acid to linolenic acid in leaf polar lipids.

収録刊行物

• Plant and cell physiology

  Plant and cell physiology 37 (5), 606-611,

  Japanese Society of Plant Physiologists

キーワード

• Linolenic acid-Nicotiana tabacum-Plastid-Wounding-w-3 fatty acid desaturase.

詳細情報

• CRID: 1543950420081219328
• NII論文ID: 110003720863
• NII書誌ID: AA0077511X
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10771907
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles