Evidence for the Involvement of Mitochondrial Respiration in Calcificationin a Marine Coccolithophorid, Emiliania huxleyi :

  • Sekino,Keisuke
    Department of Biology, Faculty of Science, Niigata University:(Present)SDS Biotech K.K.,Tsukuba Technology Center
  • Shiraiwa,Yoshihiro
    Department of Biology, Faculty of Science, Niigata University

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In the marine coccolithophorid, Emiliania huxleyi, CaCO_3 production under illumination showed a lag phase for about 3 h and then increased greatly. During the lag phase the rate of CaCO_3 production in the light was similar to that in the dark. The production of CaCO_3 in the dark was inhibited by the addition of 170μM CCCP, 1 mM KCN and 1 mM SHAM. These results suggest that a little production of CaCO_3 is supported by energy from mitochondrial respiration, but that large amount of CaCO_3 pro-duction requires photosynthesis.

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