Rapid genome changes after interspecific hybridization between Chinese Dendranthema indica×D. vestita identified by fluorescent in situ hybridization and 4', 6-diamidino-2-phenylindole
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Fluorescent in situ hybridization (FISH) signals of the 45S rDNA sites showed that Dendranthema indica had 12 signals, D. vestita had ten signals and their F_1 hybrid had nine signals instead of 11 signals. 4', 6-diamidino-2-phenylindole (DAPI) after FISH on the same metaphase chromosomes showed that D. indica had DAPI-positive bands on terminal region in 34 chromosomes, of which six chromosomes had terminal bands on both short and long arms, 11 sat-chromosomes had DAPI bands terminal on the long arm. Dendranthema vestita showed DAPI positive bands in terminal regions of 20 chromosomes of which two sat-chromosomes had DAPI bands on the long arm. The hybrid had 22 chromosomes instead of 27 chromosomes carrying DAPI-positive bands. Four chromosomes instead of three chromosomes had DAPI positive bands on both arms could be related to D. indica. Four sat-chromosomes had no DAPI-positive bands could be related to D. vestita. Combination of FISH and DAPI showed deletions in two sites of 45S rDNA and five regions of DAPI positive bands and one extra chromosome with DAPI terminal bands on both arms, which might be resulted due to translocation and recombination indicating rapid genome changes after the hybridization.