An Intersectional Hybrid in the Genus Pteris (Pteridaceae) on Okinawa Island : Evidence from Nuclear and Chloroplast DNA

• DENDA TETSUO

  Laboratory of Ecology and Systematics,Faculty of Science,University of the Ryukyus

• TOMIYAMA HAJIME

  Maehara High School

• YOKOTA MASATSUGU

  Laboratory of Ecology and Systematics,Faculty of Science,University of the Ryukyus

抄録

A putative hybrid of the fern genus Pteris was collected on Okinawa Island. It was morphologically intermediate between P. ryukyuensis (section Pteris) and P. semipinnata (section Campteria), suggesting that it might be an intersectional hybrid between them. The hybrid nature of the plant also suspected because of the abnormal morphology of the spores and sporangia. To clarify the parcentage of this putative hybrid, we analyzed the nuclear (nDNA) and chloroplast (cpDNA) DNA of all three of them. PCR analysis of the internal transcribed spacer 1 region of the nDNA indicated that the putative hybrid possessed nDNA of P. ryukyuensis. Furthermore, PCR-SSCP analysis of the intergenic spacer between psbC and trnS of cpDNA showed the putative hybrid to have the same electrophoretic pattern as P. semipinnata. Because the cpDNA is generally maternally inherited, we concluded that the putative hybrid was an intersectional hybrid between P. ryukyuensis and P. semipinnata, with the latter species functioning as the mother.

収録刊行物

• Acta Phytotaxonomica et Geobotanica

  Acta Phytotaxonomica et Geobotanica 52 (2), 159-165, 2001

  日本植物分類学会

キーワード

• internal transcribed spacer
• intersectional hybrid
• natural hybrid
• Okinawa Island
• PCR-SSCP
• Pteris ryukyuensis
• Pteris semipinnata
• ribosomal DNA

詳細情報

• CRID: 1390001205269976064
• NII論文ID: 110003733658
• NII書誌ID: AA11586265
• DOI: 10.18942/apg.kj00003256649
• ISSN: 21897042
• ISSN: 13467565
• 本文言語コード: en
• データソース種別:
  • JaLC
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可