低出力レーザーの骨に対する影響　病理組織学的および細胞生理学的検討

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  • Effects of low power laser on bone. Histropathological and cellular physiological study.
  • Histopathological and Cellular Physiological Study
  • 病理組織学的および細胞生理学的検討

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The purpose of the present study is to evaluate the effects of low power laser on bone formation.<BR>In the in vivo study, artificial bone defects in the mandible of the dogs were used to investigatethe effect of laser irradiation on the process of healing. The number of cellular elements inbone marrow, including osteoclasts in Howship's lacnae, increased on the laser-irradiation side at3 days after the operation. At 7 days, the mass of newly formed bone around the defect on thelaser-irradiation side was apparently more than that on the control side. In the later days, however, there were no differences between the two groups.<BR>In the in vitrostudy, osteoblastic cells (UMR 106) were used to investigate the effect of laserirradiation on their function. Laser irradiation increased DNA synthesis in the cells at a logarithmicphase without effect on their morphology. To exert this stimulatory effect, more than 3min/day laser irradiation was necessary in the presence of more than 5%(v/v) FCS. In both PTHand PGE2-treated cells, however, laser irradiation had no significant effect on DNA synthesis. Inaddition, laser irradiation increased alkaline phosphatase activity (per protein).<BR>These findings suggest that low power laser may stimulate the osteoblast-mediated boneformation.

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