Seminal Plasma Lactoferrin but Not Transferrin Reflects Gonadal Function in Dogs

• KIKUCHI Motohiro

  Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• MIZOROKI Shohei

  Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University

• KUBO Takashi

  Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University

• OHIWA Yoshiko

  Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• KUBOTA Miho

  Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• YAMADA Naoki

  Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• ORINO Koichi

  Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University

• OHNAMI Yohji

  Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University

• WATANABE Kiyotaka

  Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University

書誌事項

タイトル別名

• Seminal plasma lactoferrin but not transferrinreHects gonadal function in dogs

抄録

Lactoferrin purified from canine seminal plasma by a three-step chromatography procedure had a molecular mass of 75.2 kDa and cross-reacted with antiserum to equine seminal plasma lactoferrin. Seminal plasma lactoferrin concentrations were determined by a competitive enzyme-linked immunosorbent assay (ELISA) by using rabbit anti-equine lactoferrin antibody and alkaline phosphatase-labeled goat anti-rabbit IgG antibody in 14 normal dogs and found to range from 12 to 197 μg/ml, with a mean value of 77 ± 59 μg/ml (the mean ± SD). Seminal plasma transferrin concentrations were determined by a sandwich ELISA with goat antibody to canine serum transferrin and alkaline phosphatase-conjugated goat anti-canine transferrin antibody and found to range from 0.32 to 12.6 μg/m l, with a mean value of 2.44 ± 3.25 μg/m l. The lactoferrin concentration significantly correlated with the sperm concentration (r=0.7025, P<0.01), but there was no significant correlation between the seminal plasma transferrin concentration and sperm density. These results indicate that seminal plasma lactoferrin, but not transferrin, reflects gonadal function.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 65 (6), 679-684, 2003

  公益社団法人 日本獣医学会

キーワード

• canine
• lactoferrin
• seminal plasma
• sperm
• transferrin

詳細情報

• CRID: 1390282681401808896
• NII論文ID: 110003886237
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.65.679
• COI: 1:STN:280:DC%2BD3szktVehsA%3D%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 6627829
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6627829
• Web Site: http://www.jstage.jst.go.jp/article/jvms/65/6/65_6_679/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003286944
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可