Effects of 17β-estradiol, bisphenol A and tributyltin chloride on germ cells of Caenorhabditis elegans

• HOSHI Hidenobu

  Department of Veterinary Public Health, Division of Veterinary Sciences, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• KAMATA Yoichi

  Department of Veterinary Public Health, Division of Veterinary Sciences, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

• UEMURA Takashi

  Department of Veterinary Public Health, Division of Veterinary Sciences, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University

書誌事項

タイトル別名

• Effects of 17.BETA.-Estradiol, Bisphenol A and Tributyltin Chloride on Germ Cells of Caenorhabditis elegans
• Effects of 17 ベータ estradiol bisphenol A and tributyltin chloride on germ cells of Caenorhabditis elegans

抄録

Effects of a one-generation exposure to a natural estrogen, 17β-estradiol (E2), and environmental pollutants such as bisphenol A (BPA) and tributyltin chloride (TBTCL) on the number of germ cells were investigated in the hermaphrodite Caenorhabditis elegans. The eggs of gravid adult worms isolated by alkaline hypochlorite treatment were seeded on a test chemical-containing NGM (nematode growth medium) agar plate without cholesterol. After incubation for 6 days at 16°C, the germ cells of adult worms were stained with 4', 6-diamino-2-phenylindole dihydrochloride (DAPI). The staining procedure was completed within one hour and the stained germ cells were counted under a fluorescence microscope without dissection. The number of germ cells in the worms treated with E2 (10^-10-10^-6 M) and BPA (10^-9-10 ^-5 M) was significantly increased. Maximal increases were observed at 10^-8 M E2 (156 ± 15.3% of control) and 10^-5 M BPA (168 ± 20.0 % of control). TBTCL (10^-9-10^-6 M) significantly decreased the number of germ cells. The minimal decrease was observed at 10^-6 M TBTCL (30.2 ± 3.51% of control). These results indicate that changes in the number of germ cells are a sensitive indicator of the effects of chemicals on the reproductive system. Since the method described in this paper is a novel, simple, time- and money-saving bioassay, C. elegans is an excellent model with which to determine the reproductive toxicity of chemicals including environmental pollutants.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 65 (8), 881-885, 2003

  公益社団法人 日本獣医学会

キーワード

• bioassay
• <i>Caenorhabditis elegans</i>
• environmental pollutant
• germ cell
• reproductive toxicity

詳細情報

• CRID: 1390282681403043328
• NII論文ID: 110003886275
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.65.881
• COI: 1:STN:280:DC%2BD3svhtFOqtg%3D%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 6686153
• PubMed: 12951420
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6686153
• Web Site: http://www.jstage.jst.go.jp/article/jvms/65/8/65_8_881/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2004019729
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可