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本研究では,台湾における牛の血清Haptoglobin(Hp)濃度の基準値を求めた。健康な未経産牛10頭より1週間隔で1年間採材し,分娩後子宮炎と診断した牛15頭の血清Hp濃度と比較した。分娩後3週以内の健康牛11頭についても血清Hp濃度を測定した。本研究で開発した測定法による値はWestern blottingの結果とよく相関していた(r = 0.893)。10頭の未経産牛から採取した454サンプルのHp濃度は83.6±34.1mg/lであり,個体間の有意差はなかった。分娩後に繁殖障害に罹患した牛の血清Hp濃度は1133.5±627.lmg/lであり,未経産牛や正常分娩牛の値(104.6±61.0mg/l)(p<0.05)より有意に高値を示した。これらの結果より血清Hp濃度が分娩後発生する繁殖障害の指標として有用であることが示唆された。
To obtain a basal concentration of serum Haptoglobin (Hp) in cattle in Taiwan, Hp concentrations were measured from serum samples collected from 10 healthy heifers, every week for one year. The values were also compared with those collected from 15 cows diagnosed with postpartum metritis. The heifers were successfully impregnated by artificial insemination six months after the tests. Hp concentrations were also measured in the serum collected from 11 other cows within 3 weeks after parturition. The Hp assay developed in this study gave a good correlation (r=0.893) with Western blotting. The Hp concentration of 454 serum samples from the 10 heifers had a mean value of 83.6±34.1 mg/l, and there was no significant difference among individual heifers. The basal value of Hp in heifers was calculated as less than 73.6 mg/I. No significant difference in Hp concentration was observed among the 10 heifers during cold and warm seasons (19.8±2.2℃ vs 27.3±l.4℃), or before and after pregnancy. The mean serum Hp concentration from cows suffering from postpartum reproductive disorders was 1133.5±627.1 mg/l, which was significantly greater than the serum of healthy heifers and postpartum cows (104.6±61.0 mg/l) (P<0.05). These results demonstrate that Hp concentration may be a useful indicator for cows with postpartum reproductive disorders.
