The Effect of Polysaccharides and Carboxymethylcellulose Combination to Prevent Intraperitoneal Adhesion and Abscess Formation in a Rat Peritonitis Model

• BAE Jae-sung

  College of Veterinary Medicine, Kyungpook National University

• JIN Hee-kyung

  College of Veterinary Medicine, Kyungpook National University

• JANG Kwang-ho

  College of Veterinary Medicine, Kyungpook National University

書誌事項

タイトル別名

• Effect of Polysaccharides and Carboxymethylcellulose Combination to Prevent Intraperitoneal Adhesion and Abscess Formation in a Rat Peritonitis Model

抄録

Polysaccharides isolated from fungi, Phellinus spp. is well-known material with anti-tumor and anti-inflammatory properties. We have assessed the adhesion- and abscess-reducing capacity of carboxymethylcellulose (CMC) and polysaccharides from Phellinus spp. combination in a rat peritonitis model. In 72 Sprague-Dawley rats, experimental peritonitis was induced by means of the cecal ligation and puncture model (CLP). After 24 hr, the abdomen was reopened and the ligated cecum was resected. Peritoneal fluid samples were taken for microbiological examination. Rats were randomly assigned to 6 groups: ringer lactate solution (RL group), polysaccharides from Phellinus gilvus (PG group) and Phellinus linteus (PL group), carboxymethylcellulose (CMC group), and their combinations (PG+CMC and PL+CMC groups). Adhesions and abscesses were noted at day 7 after CLP. RT-PCR assay for urokinase-type plasminogen activator (uPA), its cellular receptor (uPAR), and tumor necrosis factor (TNF)-α was performed to assess the cecal tissue. Microbiological examination showed polymicrobial bacterial peritonitis. Adhesion formation was significantly reduced in PG+CMC and PL+CMC groups (P<0.05). The incidence of abscesses was reduced in all treated groups except the RL group (P<0.05). uPA, uPAR, and TNF-α mRNA were highly expressed in the PG+CMC and PL+CMC groups, as compared to the RL group. We concluded that the combination of polysaccharides and CMC had significant adhesion- and abscess-reducing effects compared with their single treatment and the effects may act by modifying the fibrinolytic capacity of uPA, uPAR and TNF-α produced from activated macrophages in a rat peritonitis model.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 66 (10), 1205-1211, 2004

  公益社団法人 日本獣医学会

キーワード

• adhesion
• peritonitis
• <i>Phellinus</i> spp.
• polysaccharides
• rat

詳細情報

• CRID: 1390282681400768128
• NII論文ID: 110003886340
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.66.1205
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 7130035
• PubMed: 15528850
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7130035
• Web Site: http://www.jstage.jst.go.jp/article/jvms/66/10/66_10_1205/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005021379
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可