乳房炎乳由来微生物のラクトフェリンに対する感受性

• LEE Nai-Yuan

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University

• KAWAI Kazuhiro

  Seibu Veterinary Clinical Center

• NAKAMURA Ichiro

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University

• TANAKA Tetsuya

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University

• KUMURA Haruto

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University

• SHIMAZAKI Kei-ichi

  Dairy Science Laboratory, Department of Animal Product Science, Graduate School of Agriculture, Hokkaido University

書誌事項

タイトル別名

• Susceptibilities against Bovine Lactoferrin with Microorganisms Isolated from Mastitic Milk

抄録

Antibacterial effects of bovine lactoferrin were studied in vitro against microorganisms isolated from mastitic milk in Tokachi area, Hokkaido, Japan. Microorganisms isolated were Eschelichia coli (11 isolates), Klebsiella pneumoniae (5 isolates), enterococci (8 isolates), Staphylococcus aureus (10 isolates), coagulase negative staphylococci (CNS, 13 isolates), streptococci (11 isolates), Prototheca zopfii (7 isolates) and yeast-like fungi (9 isolates). Lactoferrin has been known as a multifunctional protein and its antimicrobial effect is one of the most essential function of it. In order to compare their susceptibilities against lactoferrin, the minimal inhibitory concentration values were estimated by a microplate assay method using 96-well microplate, which involved measuring the optical density of the cultures. Prototheca zopfii was highly sensitive to bovine lactoferrin and complete inhibition of this microorganism was observed even at the low concentration of 7 μg/ml. On the other hand, E. coli and enterococci showed resistance against lactoferrin action and staphylococci showed strain-dependent resistance.<br>

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 66 (10), 1267-1269, 2004

  公益社団法人 日本獣医学会

キーワード

• antimicrobial activity
• lactoferrin
• mastitic milk

詳細情報

• CRID: 1390001206424065792
• NII論文ID: 110003886352
• NII論文ID: 130000448741
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.66.1267
• ISSN: 13477439
• ISSN: 09167250
• HANDLE: 2115/39894
• NDL書誌ID: 7130143
• PubMed: 15528862
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I7130143
• Web Site: http://www.jstage.jst.go.jp/article/jvms/66/10/66_10_1267/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2005021386
• 本文言語コード: en
• データソース種別:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可