Prevalence of Porcine Endogenous Retrovirus in Domestic Pigs in Japan and Its Potential Infection in Dogs Xenotransplanted with Porcine Pancreatic Islet Cells

DOI Web Site Web Site PubMed 被引用文献4件 参考文献33件
  • EDAMURA Kazuya
    Laboratories of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
  • NASU Koko
    Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
  • IWAMI Yukiko
    Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University
  • NISHIMURA Ryohei
    Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • OGAWA Hiroyuki
    Laboratories of Veterinary Emergency Medicine, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • SASAKI Nobuo
    Veterinary Surgery, Graduate School of Agricultural and Life Sciences, The University of Tokyo
  • OHGAWARA Hisako
    Division of Cell Replacement and Regenerative Medicine, Medical Research Institute, School of Medicine, Tokyo Women's Medical University

この論文をさがす

抄録

The prevalence of porcine endogenous retrovirus (PERV) proviral DNA among various pig breeds raised in Japan was investigated by polymerase chain reaction (PCR). Moreover, potential infection of PERV was investigated by PCR and reverse transcriptase-polymerase chain reaction (RT-PCR) in experimentally induced diabetic dogs (n=5) implanted with the diffusion chamber type bio-artificial endocrine pancreas (Bio-AEP) containing porcine pancreatic endocrine (PE) cells. No immunosupplessant was used after the transplantation. PERV gag, pol, env-A and env-B genes were detected in any pigs examined. In two of three Landrace breeds, env-C gene was absent. PERV proviral DNAs and viral RNAs were also detected from the cultured porcine PE-cells. In the peripheral blood mononuclear cells and the spleen obtained at 6, 30, 32, 36, 79 weeks of xenotransplantation in dogs, however, no evidence of microchimerism, infection and viremia were confirmed. These results suggested that the risk of PERV infection through xenotransplantation of Bio-AEP containing porcine islet cells without immunosuppressants may be quite low.<br>

収録刊行物

被引用文献 (4)*注記

もっと見る

参考文献 (33)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ