Acquisition of Quinolone Resistance and Point Mutation of the gyrA Gene in Campylobacter jejuni Isolated from Broilers and in vitro-Induced Resistant Strains
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- CHUMA Takehisa
- Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University
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- MAEDA Takatoshi
- Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University
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- NIWA Hidekazu
- Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University
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- OKAMOTO Karoku
- Department of Veterinary Public Health, Faculty of Agriculture, Kagoshima University
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A dramatic rise in the number of resistant Campylobacter to quinolones has been documented in human patients and domestic animals. In this study, the mechanism of acquisition of quinolone resistance was studied by detecting point mutations in the gyrA gene of Campylobacter strains obtained from broilers and strains with in vitro-induced resistance. The minimal inhibitory concentrations (MICs) of norfloxacin (NFLX) and ofloxacin (OFLX) for the strains that had no point mutation were slightly increased from the source strain (Campylobacter jejuni ATCC 33560). The MICs of nalidixic acid (NA), NFLX, and OFLX for the strains that had the point mutation at Thr-86 were 100 or 200 μg/ml, 50 μg/ml, and 25 μg/ml, respectively. The MIC of NA for the strain that had a point mutation at Asp-90 higher than those for the strains that had the point mutation at Thr-86, but the MICs of NFLX and OFLX were relatively lower than those for the strains that had point mutation at Thr-86. These findings suggest that the degree of antimicrobial resistance against NA, NFLX, and OFLX in the in vitro-induced C. jejuni strains was associated with the location of the point mutation in gyrA. On the other hand, a point mutation in all seven resistant strains isolated from broilers was located only at Thr-86, while the MICs of the three quinolones varied in each wild strain. This suggests that another mechanism might also be involved in the acquisition of quinolone resistance in C. jejuni wild strains.<br>
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 66 (2), 155-160, 2004
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206426672640
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- NII論文ID
- 110003886372
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- NII書誌ID
- AA10796138
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6863965
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- PubMed
- 15031543
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 使用不可