Controlling the Orientation of Purple Membrane Fragments on an Air/Water Interface by a New Method of Direct Electric Field Application during Purple Membrane Spreading.
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- Sugiyama Yukihiro
- Tsukuba Research Center, Sanyo Electric Co., Ltd., 2–1 Koyadai, Tsukuba, Ibaraki 305, Japan
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- Inoue Takakazu
- Tsukuba Research Center, Sanyo Electric Co., Ltd., 2–1 Koyadai, Tsukuba, Ibaraki 305, Japan
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- Ikematsu Mineo
- Tsukuba Research Center, Sanyo Electric Co., Ltd., 2–1 Koyadai, Tsukuba, Ibaraki 305, Japan
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- Iseki Masahiro
- Microelectronics Research Center, Sanyo Electric Co., Ltd., 180 Ohmori, Anpachi–cho, Anpachi–gun, Gifu 503–01, Japan
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- Sekiguchi Tatsuhiko
- Tsukuba Research Center, Sanyo Electric Co., Ltd., 2–1 Koyadai, Tsukuba, Ibaraki 305, Japan
書誌事項
- タイトル別名
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- Controlling the Orientation of Purple M
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The orientation of purple membrane (PM) fragments on an air/water interface was controlled by a direct electric field applied across the interface during spreading of a PM solution. We call this the direct electric field application Langmuir-Blodgett (LB) technique, and found it effective in increasing the population of preferentially oriented PM fragments at the interface. Using this technique with an electric field exceeding 4 kV/cm and an initial coverage C i of 0.2 (i.e., the ratio of the surface area of the air/water interface covered by PM fragments to the total surface area of the interface before the PM was spread over the interface), we produced a PM LB film in which all the constituent PM fragments were preferentially oriented. We successfully demonstrated that this technique is a powerful tool for producing LB films in which proteins are perfectly oriented (i.e., all the constituent PM fragments are preferentially oriented).
収録刊行物
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- Japanese Journal of Applied Physics
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Japanese Journal of Applied Physics 36 (9A), 5674-5679, 1997
The Japan Society of Applied Physics
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詳細情報 詳細情報について
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- CRID
- 1390282681228691712
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- NII論文ID
- 110003906129
- 210000041718
- 130004524017
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- NII書誌ID
- AA10457675
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- COI
- 1:CAS:528:DyaK2sXms1Gjsb4%3D
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- ISSN
- 13474065
- 00214922
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- NDL書誌ID
- 4307529
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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