Antigenic Differentiation of Turkey Rhinotracheitis Virus Strains Using Monoclonal Antibodies and Polyclonal Antisera.
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- OBI Takeshi
- Division of Veterinary Microbiology, Kyoto Biken Laboratories
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- KOKUMAI Norihide
- Division of Veterinary Microbiology, Kyoto Biken Laboratories
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- IBUKI Akiko
- Division of Veterinary Microbiology, Kyoto Biken Laboratories
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- TAKUMA Hiroshi
- Division of Veterinary Microbiology, Kyoto Biken Laboratories
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- TANAKA Masayuki
- Division of Veterinary Microbiology, Kyoto Biken Laboratories
書誌事項
- タイトル別名
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- Antigenic differentiation of turkey rhi
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抄録
Monoclonal antibodies (mAbs) were prepared against the 8597/CV94 strain of turkey rhinotracheitis virus (TRTV). These mAbs were used to investigate antigenic relationships among three strains (8597/CV94, 1162/92 and CVL14/1 strain) of TRTV, together with polyclonal chicken and rabbit antisera to 8597/CV94 strain, and guinea pig antisera to each of the three strains. Thirty mAbs to the glycoprotein (G:3 clones), fusion (F1:6 clones), phosphorylated (P:6 clones), nucleocapsid (N:12 clones), and matrix (M:3 clones) proteins of viral antigen were obtained by cell fusion. Among these, two mAbs to F1 protein showed virus neutralizing activity. The results of ELISA test indicated that some mAbs only reacted to the 8597/CV94 strain, some reacted to 8597/CV94 and 1162/92 strains, and others reacted to all three viral strains. In neutralization tests with the three virus strains, polyclonal chicken and rabbit antisera against the 8597/CV94 strain showed the same antibody titers. Results with four neutralizing mAbs including two previously reported mAbs [Ref. 21] indicated the titers of two mAbs (Pn2-2E and Pn3-2F) to 8597/CV94 were much higher than those to the other two viral strains. No differences were observed in the titers of the other two mAbs (Pn01-8E and Pn06-4D) against any viral strains. In cross-neutralization tests with polyclonal guinea pig antisera, there was some variations among viral strains. This work demonstrated that the Japanese isolate 8597/CV94 of TRTV is somewhat different in antigenicity from two British isolates from chickens and turkeys.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 59 (9), 795-799, 1997
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681403877248
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- NII論文ID
- 110003915244
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DyaK1c%2Fgt1SqtA%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 4305192
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- PubMed
- 9342704
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可