書誌事項
- タイトル別名
-
- Histamine Receptors on Bovine Peripheral Blood Lymphocytes.
- 牛末梢血リンパ球におけるヒスタミンレセプター〔英文〕
- ウシ マッショウケツ リンパキュウ ニ オケル ヒスタミン レセプター エイブ
この論文をさがす
抄録
Histamine receptors on bovine peripheral blood lymphocytes (PBL) were detected by three different methods: a rosetting technique, binding to histamine-bearing Sepharose beads and immunofluorescence staining. The rosetting technique used histamine-rabbit serum albumin (H-RSA) conjugated to bovine red blood cells to detect histamine receptors and this showed that 10.8% of bovine PBL were positive. A method using H-RSA conjugate coupled Sepharose beads also detected histamine receptor bearing PBL but was not quantitative. The indirect immunofluorescence method, by which the subpopulation of histamine receptor bearing lymphocytes can be easily double stained to concurrently identify the B cell marker, revealed that PBL, the B cell and T cell fraction of bovine PBL contained 18.4, 52.8 and 9.3% histamine receptor bearing cells, respectively. This method was found to be more stable and more easily quantifiable than the other two methods. Blocking tests using the histamine H1 receptor antagonist diphenhydramine and the histamine H2 receptor antagonist cimetidine suggested that bovine PBL have both H1 and H2 receptors on their surfaces. Addition of histamine into cultures of PBL at the concentration range 10-6 to 10-3 M suppressed the response of PBL to the mitogen phytohemagglutinin. The histamine induced suppression of mitogenesis could be reduced partially by the H2 receptor antagonist cimetidine, but not by the H1 antagonist diphenhydramine . It is possible that histamine induced suppression of PBL mitogenesis was mediated by H2 receptors on T cells.
収録刊行物
-
- The Journal of Veterinary Medical Science
-
The Journal of Veterinary Medical Science 56 (1), 45-49, 1994
公益社団法人 日本獣医学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681401700224
-
- NII論文ID
- 130003571874
- 110003915988
-
- NII書誌ID
- AA10796138
-
- COI
- 1:CAS:528:DyaK2cXkt1OmtLY%3D
-
- ISSN
- 13477439
- 09167250
-
- NDL書誌ID
- 3869795
-
- PubMed
- 7911336
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可