Pasteurella multocida Toxin and Bordetella bronchiseptica Dermonecrotizing Toxin Elicit Similar Effects on Cultured Cells by Different Mechanisms.
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- OHNISHI Takahiro
- Department of Bacterial Toxinology, Research Institute for Microbial Disease, Osaka University
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- HORIGUCHI Yasuhiko
- Department of Bacterial Toxinology, Research Institute for Microbial Disease, Osaka University
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- MASUDA Minako
- Department of Bacterial Toxinology, Research Institute for Microbial Disease, Osaka University
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- SUGIMOTO Nakaba
- Department of Bacterial Toxinology, Research Institute for Microbial Disease, Osaka University
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- MATSUDA Morihiro
- Department of Bacterial Toxinology, Research Institute for Microbial Disease, Osaka University
書誌事項
- タイトル別名
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- Pasteurella multocida toxin and Bordete
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We compared the effects of Pasteurella multocida toxin (PMT) with Bordetella bronchiseptica dermonecrotizing toxin (DNT) at a cellular level under same conditions. Both PMT and DNT cause actin stress fiber formation in MC3T3-E1 cells which is known to be regulated by the small GTP-binding protein Rho. DNT induced mobility shifts of Rho on SDS-polyacrylamide gel electrophoresis, indicating direct modification as reported elsewhere. In contrast, no alternations in the electrophoretic mobility of Rho were found in lysates from PMT-treated cells. PMT but not DNT increased the intracellular level of inositol phosphates, indicating the elevation of phospholipase C (PLC) activity in the PMT-treated cells. These results indicate that PMT does not have Rho as a target but activates PLC. The formation of actin stress fiber by PMT seems to be stimulated through the indirect activation of Rho, which resides downstream of PLC. PMT and DNT seem to elicit similar toxic effects, at least in part, through the activation of Rho.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 60 (3), 301-305, 1998
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206424517504
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- NII論文ID
- 110003918426
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DyaK1cXisVKisLo%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 4437736
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- PubMed
- 9560776
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可