Monoclonal Antibody TSd-1 is Specific to Elongating and Matured Spermatids in Testis of Common Tree Shrew(Tupaia glis).

• MAEDA Seishi

  Department of Veterinary Anatomy, The University of Tokyo

• NAKAMUTA Nobuaki

  Department of Veterinary Anatomy, The University of Tokyo

• NAM Sang-Yoon

  Department of Veterinary Anatomy, The University of Tokyo

• KIMURA Junpei

  Department of Veterinary Anatomy, Nihon University

• ENDO Hideki

  Department of Zoology, National Science Museum

• RERKAMNUAYCHOKE Worawut

  Department of Veterinary Anatomy, Kasetsart University

• KUROHMARU Masamichi

  Department of Veterinary Anatomy, The University of Tokyo

• YAMADA Junzo

  Department of Veterinary Anatomy, Obihiro University of Agriculture and Veterinary Medicine

• HAYASHI Yoshihiro

  Department of Veterinary Anatomy, The University of Tokyo

• NISHIDA Takao

  Laboratory of Anatomy and Physiology, Nihon University

書誌事項

タイトル別名

• Monoclonal antibody TSd-1 is specific t

抄録

The monoclonal antibody (MAb), named TSd-1, specific to spermatogenic cells of the common tree shrew (Tupaia glis) was established and characterized using immunohistochemistry and immunoblotting. MAb TSd-1 reacted with elongating and matured spermatids in a stage-dependent manner. TSd-1 recognized a 94 kilodalton (kDa) peptide in the plasma membrane and cytosol. Additionally, an extremely weak 107 kDa band was detected only in the cytosol. The reactions were not detected in round spermatids. In elongating Stage VI spermatids, the plasma membrane and the granular structure within the cytoplasm were intensely positive, and most intense after the appearance of new round spermatids in the lower layer (Stage I). The reactions were observed neither in the other organs of the common tree shrew nor in the testes of other animals, indicating that TSd-1 antigen is specific to the spermatogenic cells of the common tree shrew, and may act on elongating or matured spermatids.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 60 (3), 345-349, 1998

  公益社団法人 日本獣医学会

キーワード

• monoclonal antibody
• spermatogenesis
• spermatozoa
• tree shrew

詳細情報

• CRID: 1390282681401206656
• NII論文ID: 110003918434
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.60.345
• COI: 1:CAS:528:DyaK1cXisVKisLg%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 4437743
• PubMed: 9560784
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4437743
• Web Site: http://www.jstage.jst.go.jp/article/jvms/60/3/60_3_345/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1998143331
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可