Seroprevalence and Field Isolation of Bovine Immunodeficiency Virus.
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- MEAS Sothy
- Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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- KABEYA Hidenori
- Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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- YOSHIHARA Shinpei
- Minami Sorachi Agricultural Mutual Aid Association
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- OHASHI Kazuhiko
- Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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- MATSUKI Shigeyuki
- Tokachi Livestock Hygiene Service Center of Hokkaido Prefecture
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- MIKAMI Yuuji
- Tokachi Livestock Hygiene Service Center of Hokkaido Prefecture
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- SUGIMOTO Chihiro
- Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
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- ONUMA Misao
- Laboratory of Infectious Diseases, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University
Bibliographic Information
- Other Title
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- Seroprevalence and Field Isolation of B
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Abstract
A seroprevalence study of bovine lentivirus, known as bovine immunodeficiency virus (BIV), was conducted in 12 different dairy herds in Hokkaido, where some herds were a high prevalence of bovine leukemia virus (BLV) infection. Amongst 611 cattle, 28.6% of cattle were BLV-seropositive, and 11.7% of cattle were seropositive for BIV, while 4.2% of cattle were seropositive for both BIV and BLV. For the isolation of BIV, 19 samples of peripheral blood mononuclear cells (PBMC) and one sample of milk-derived leukocytes were prepared from BIV-seropositive cows. These PBMC and leukocyte preparations were then co-cultivated with cc81 cells, a cat cell line transformed by mouse sarcoma virus. BIV was isolated from 17 PBMC and one milk-derived leukocyte samples. The isolated viruses showed slow replication and syncytia formation. Major core antigen, p26 from these isolates were reacted with anti-BIV (American isolate R-29) serum. In addition, proviral DNA was detected in blood and milk samples by nested polymerase chain reaction and subsequent Southern blot hybridization. Nucleotide sequence analysis of the amplified pol gene products showed its 99.0 to 99.7% homology to that of BIV R-29. These results indicate that the Japanese BIV isolates appear to be antigenically and genetically similar to the American R-29. Since BIV was isolated from milk samples, BIV could possibly be transmitted through milk. This is the first report of BIV isolation in Japan.
Journal
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- Journal of Veterinary Medical Science
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Journal of Veterinary Medical Science 60 (11), 1195-1202, 1998
JAPANESE SOCIETY OF VETERINARY SCIENCE
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Details 詳細情報について
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- CRID
- 1390001206426102528
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- NII Article ID
- 110003918579
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- NII Book ID
- AA10796138
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- COI
- 1:CAS:528:DyaK1MXhsVyisw%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL BIB ID
- 4609146
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- PubMed
- 9853299
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed