低温細菌群の発育温度に関する研究 : 生乳および生肉由来 Pseudomonas の種々の培養温度における発育態度について

DOI 日本農学文献記事索引 Web Site Web Site PubMed 被引用文献1件

書誌事項

タイトル別名
  • Studies on the Growth Temperature of Psychrotrophic Bacteria : Growth at Various Incubation Temperatures of Psychrotrophic Pseudomonas Originated from Raw Milk and Raw Meat
  • テイオン サイキングン ノ ハツイク オンド ニカンスルケンキュウ セイニュウ

この論文をさがす

抄録

The maximum, minimum and optimum growth temperatures of 50 psychrotrophic Pseudomonas strains (20 from raw milk, 10 from fresh pork and 20 from frozen veal) and 3 strains of mesophilic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli) were examined by stationary cultivation at 12 different temperatures at intervals of about 5°C between -8°C and 40°C, and by shaking temperature gradient Cultivation at 30 different temperatures at intervals of about 1.5°C between 0°C and 45°C. The growth rates at various incubation temperatures were measured by optical density in trypticase soy broth (BBL) culture, using an electric photometer with 530 nm filter. Of the 50 Pseudomonas strains examined, 42 grew at -8°C after 7- to 15-week incubation in a non-freezing broth (trypticase soy broth with 5% glycerin added), and 47 strains at -5°C after 3- to 4-week incubation. 0f 3 strains unable to grow at -5°C for 10 weeks, one grew at -2°C for 3 weeks, and two at 1.5°C for 4 to 7 days. The maximum growth temperatures of the 50 strains examined ranged from 30 to 40.5°C, or mostly from 33 to 37°C. The optimum growth temperatures of the 50 strains ranged from 25.5 to 34.5°C, but those of 37 strains of them were 30°C or below. It is preferable that all the 50 Psepdomonas strains are called "psychrotrophs" rather than "psychrophiles", according to the following results. Although two of the 50 strains were unable to grow at 0°C within 2 weeks, all the 50 strains grew at temperatures lower than 1.5°C within a week, and their optimum growth temperatures were between 25.5 and 34.5°C. On the other hand, the lowest growth temperatures of the 3 mesophilic strains tested were between 4.5 and 7.5°C by gradient cultivation. These strains showed no growth at 5°C for 14 days by stationary cultivation. Their highest growth temperatures were over 45°C. Their optimum growth temperatures were 37.5 and 40.5°C.

収録刊行物

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ