Rapid and Direct Detection of Clostridium chauvoei by PCR of the 16S-23S rDNA Spacer Region and Partial 23S rDNA Sequences.
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- SASAKI Yoshimasa
- National Veterinary Assay Laboratory
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- YAMAMOTO Kinya
- National Veterinary Assay Laboratory
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- KOJIMA Akemi
- National Veterinary Assay Laboratory
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- TETSUKA Yukie
- Tochigi Pre. Kenhoku Animal Hygiene Center
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- NORIMATSU Mari
- Institute for Animal Health
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- TAMURA Yutaka
- National Veterinary Assay Laboratory
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抄録
Clostridium chauvoei causes blackleg, which is difficult to distinguish from the causative clostridia of malignant edema. Therefore, a single-step PCR system was developed for specific detection of C. chauvoei DNA using primers derived from the 16S-23S rDNA spacer region and partial 23S rDNA sequences. The specificity of the single-step PCR system was demonstrated by testing 37 strains of clostridia and 3 strains of other genera. A 509 bp PCR product, which is a C. chauvoei-specific PCR product, could be amplified from all of the C. chauvoei strains tested, but not from the other strains. Moreover, this single-step PCR system specifically detected C. chauvoei DNA in samples of muscle from mice 24 hr after inoculation with 100 spores of C. chauvoei, and in clinical materials from a cow affected with blackleg. These results suggest that our single-step PCR system may be useful for direct detection of C. chauvoei in culture and in clinical materials from animals affected with blackleg.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 62 (12), 1275-1281, 2000
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206425928192
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- NII論文ID
- 110003920511
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD3MXltVSmtg%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 5608512
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- PubMed
- 11193343
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可