Purification and Quantification of Lactoferrin in Equine Seminal Plasma.
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- INAGAKI Masami
- Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- KIKUCHI Motohiro
- Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- ORINO Koichi
- Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- OHNAMI Yohji
- Laboratory of Theriogenology, School of Veterinary Medicine and Animal Sciences, Kitasato University
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- WATANABE Kiyotaka
- Laboratory of Biochemistry, School of Veterinary Medicine and Animal Sciences, Kitasato University
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Lactoferrin with a molecular mass of 80 kDa was purified from equine seminal plasma by heparin-Agarose affinity chromatography and Sephacryl S-200 gel filtration. Purified lactoferrin was found to be highly homogeneous on the bases of its migration as a single band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis and of the monospecificity of rabbit antibodies to the purified protein in immunoblotting of seminal plasma proteins. A sandwich enzyme-linked immunosorbent assay was developed for quantifying lactoferrin in equine seminal plasma. Seminal plasma lactoferrin concentrations in 23 normal stallions ranged from 42 to 453 μg/ml, with a mean value of 157 ± 118 μg/ml (S.D.).
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 64 (1), 75-77, 2002
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390001206427443072
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- NII論文ID
- 130000449489
- 110003920823
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- NII書誌ID
- AA10796138
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- COI
- 1:CAS:528:DC%2BD38XhvFGltLo%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 6052476
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- PubMed
- 11853152
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可