Supplementation of Heterologous Complement Induces Anti-Thy-1.1 Nephritis in the Mongolian Gerbil (Meriones unguiculatus).

• SHICHINOHE Kazuhiro

  Department of Laboratory Animal Science, Nippon Medical School

• SHIMIZU-SUGANUMA Masumi

  Department of Laboratory Animal Science, Nippon Medical School

• GHAZIZADEH Mohammad

  Department of Molecular Pathology, Institute of Gerontology, Nippon Medical School

• ISHIZAKI Masamichi

  Department of Pathology, Nippon Medical School

抄録

Anti-Thy-1.1 nephritis in the rat is a popular experimental model for mesangial proliferative glomerulonephritis (GN). This model is characterized by direct binding of anti-Thy-1.1 antibody with Thy-1.1 antigen expressed on mesangial cells (MCs) of glomeruli in the rat. A single injection of anti-rat thymocyte serum (ARTS) results in GN with proteinuria and extensive mesangiolysis. Development of mesangiolysis and proteinuria are complement-dependent. We previously demonstrated Thy-1.1 antigen, similar to the rat, in thymocytes, brain cells and MCs of the kidney in the Mongolian gerbil (MG). In this study, we attempted to develop a MG nephritis model, but an injection of ARTS did not induce GN. An additional injection of guinea pig serum as a complement after ARTS injection resulted in anti-Thy-1.1 nephritis in MG. Degeneration of MCs and neutrophil infiltration were observed 1 hr after GP serum injection. Mesangiolysis and fibrin exudation occurred 12 hr after the injection and MC proliferation was apparent 7 days after the injection. In the complement-dependent hemolytic test, MG serum could not hemolyze sheep erythrocytes. These results suggested low activity, or depletion of some factors, in complements of MG serum.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 64 (6), 463-467, 2002

  公益社団法人 日本獣医学会

キーワード

• complement
• Mongolian gerbil
• nephritis
• Thy-1.1 antigen

詳細情報

• CRID: 1390001206425506944
• NII論文ID: 130000449286
• NII論文ID: 110003920878
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.64.463
• COI: 1:STN:280:DC%2BD38zosVanug%3D%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 6200413
• PubMed: 12130828
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6200413
• Web Site: http://www.jstage.jst.go.jp/article/jvms/64/6/64_6_463/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003053657
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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