Analysis of Serum Cytokine Profile in a Holstein Heifer with Leukocyte Adhesion Deficiency Which Survived for Long Period.

• NAGAHATA Hajime

  Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

• HAGIWARA Katsuro

  Department of Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University

• HIGUCHI Hidetoshi

  Department of Animal Health, School of Veterinary Medicine, Rakuno Gakuen University

• KIRISAWA Rikio

  Department of Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University

• IWAI Hiroshi

  Department of Veterinary Microbiology, School of Veterinary Medicine, Rakuno Gakuen University

抄録

Serum cytokine levels and their expression of mRNA on neutrophils from a bone marrow (BM) transplanted heifer with leukocyte adhesion deficiency were evaluated. The clinical condition of the affected heifer was relatively stable after BM-transplantation. Persistent hyper γ-globulinemia and increased serum immunoglobulin G (IgG) concentrations were monitored longitudinally. The concentration of interleukin (IL)-1β in serum from the affected heifer ranged from 15.8 to 321.7 ng/ml, and maximum concentration occurred at the time which coincided with peak IL-6. Serum levels of IL-6 ranged from 0.32 to 27.9 ng/m l, and they appeared to be associated with the increment of serum IgG in the affected heifer. mRNAs for IL-1β, IL-6, IL-8 and granulocyte and macrophage colony stimulating factor (GM-CSF) were increased in neutrophils from the affected heifer compared to controls. Persistent hyper γ-globulinemia of the affected heifer appeared to be associated with enhanced mRNA expression for IL-6 and its serum levels. These findings suggest that humoral immunity of the affected heifer is activated and the production of neutrophils appears to be enhanced under the incapability of β₂ integrin-mediated functions of phagocytic cells.

収録刊行物

• Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ

  Ｔｈｅ　Ｊｏｕｒｎａｌ　ｏｆ　Ｖｅｔｅｒｉｎａｒｙ　Ｍｅｄｉｃａｌ　Ｓｃｉｅｎｃｅ 64 (8), 683-687, 2002

  公益社団法人 日本獣医学会

キーワード

• BLAD
• IL-1b
• IL-6
• IL-8

詳細情報

• CRID: 1390001206425992064
• NII論文ID: 130000449243
• NII論文ID: 110003920898
• NII書誌ID: AA10796138
• DOI: 10.1292/jvms.64.683
• COI: 1:CAS:528:DC%2BD38XnsFOiu78%3D
• ISSN: 13477439
• ISSN: 09167250
• NDL書誌ID: 6275430
• PubMed: 12237512
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6275430
• Web Site: http://www.jstage.jst.go.jp/article/jvms/64/8/64_8_683/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2003053177
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可